Differential expression of KCNQ1 K+ channel in tubular cells of frog kidney

  • S. Ćirović University of Belgrade, .
  • J. Marković-Lipkovski | acal@matf.bg.ac.rs University of Belgrade, .
  • J. Todorović University of Belgrade, .
  • J. NeÅ¡ović-Ostojić University of Belgrade, .
  • M. Jović Military Medical Academy, Belgrade, .
  • S. Ilić Military Medical Academy, Belgrade, .
  • S. Tatić University of Belgrade, .
  • D. ÄŒemerikić University of Belgrade, .

Abstract

The aim of this study was to evaluate KCNQ1 K+ channel expression in the frog kidney of Rana esculenta. KCNQ1 K+ channel, also known as KvLQT1, is the pore forming a-subunit of the IKs K+ channel, a delayed rectifier voltage-gated K+ channel, which has an important role in water and salt transport in the kidney and gastrointestinal tract. The expression of KCNQ1 K+ channel along tubular epithelium differs from species to species. In the present study the expression of KCNQ1 K+ channel in the frog kidney has been demonstrated by immunohistochemistry. The presence of KCNQ1 K+ channel was demonstrated in the epithelial cells of distal convoluted tubule and collecting duct. However, the pattern of expression of KCNQ1 K+ channel differs between distal convoluted tubules and collecting duct. All epithelial cells of distal convoluted tubules revealed basolateral expression of KCNQ1 K+ channel. On the contrary, only the single cells of collecting duct, probably intercalated cells, showed diffuse cell surface staining with antibodies against KCNQ1 K+ channel. These findings suggest that KCNQ1 K+ channel has cell-specific roles in renal potassium ion transport.

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Author Biography

J. Marković-Lipkovski, University of Belgrade
Institute of Pathology, Faculty of Medicine
Published
2010-02-08
Section
Original Papers
Keywords:
KCNQ1 K channel, Rana esculenta, frog kidney, immunohistochemistry
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How to Cite
Ćirović, S., Marković-Lipkovski, J., Todorović, J., Nešović-Ostojić, J., Jović, M., Ilić, S., Tatić, S., & Čemerikić, D. (2010). Differential expression of KCNQ1 K+ channel in tubular cells of frog kidney. European Journal of Histochemistry, 54(1), e7. https://doi.org/10.4081/ejh.2010.e7