Discrete foci containing RNAse A are found in nucleoli of HeLa cells after aging in culture

  • M. Costanzo University of Pavia, Italy.
  • B. Cisterna University of Pavia, Italy.
  • O. O. Zharskaya Russian Academy of Sciences, Russian Federation.
  • O. V. Zatsepina Russian Academy of Sciences, Russian Federation.
  • M. Biggiogera | marcobig@unipv.it University of Pavia, Italy.

Abstract

We have studied by means of ultrastructural immunocytochemistry the localization of RNase A in nuclei of HeLa cells in control conditions and following cell ageing in culture. We have found that roundish, electron dense foci, which contain a significant amount of RNAse A, can be detected within nucleoli of aged cells. These bodies also contain RNA and lack ribosomal S3 proteins, and may represent either simple storage sites or areas where RNA degradation takes place.

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Author Biographies

M. Costanzo, University of Pavia
Laboratorio di Biologia Cellulare e Neurobiologia, Dipartimento di Biologia Animale
B. Cisterna, University of Pavia
Laboratorio di Biologia Cellulare e Neurobiologia, Dipartimento di Biologia Animale
O. O. Zharskaya, Russian Academy of Sciences
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,
O. V. Zatsepina, Russian Academy of Sciences
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,
M. Biggiogera, University of Pavia
Laboratorio di Biologia Cellulare e Neurobiologia, Dipartimento di Biologia Animale
Published
2011-04-13
Section
Letters to the Editor
Supporting Agencies
CNR-RAS Scientific collaborative project, Russian Foundation for Basic Research (grant 08-04-00854).
Keywords:
electron microscopy, immunocytochemistry
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How to Cite
Costanzo, M., Cisterna, B., Zharskaya, O. O., Zatsepina, O. V., & Biggiogera, M. (2011). Discrete foci containing RNAse A are found in nucleoli of HeLa cells after aging in culture. European Journal of Histochemistry, 55(2), e15. https://doi.org/10.4081/ejh.2011.e15