Immunohistochemical evidence: testicular and scented glandular androgen synthesis in muskrats (Ondatra zibethicus) during the breeding season

  • Q. Li Beijing Forestry University, China.
  • J. Weng Beijing Forestry University, China.
  • H. Zhang Beijing Forestry University, China.
  • L. Lu Beijing Forestry University, China.
  • X. Ma Beijing Forestry University, China.
  • Q. Wang Beijing Forestry University, China.
  • H. Cao Beijing Forestry University, China.
  • S. Liu Beijing Forestry University, China.
  • M. Xu Beijing Forestry University, China.
  • Q. Weng | qiangweng@bjfu.edu.cn Beijing Forestry University, China.
  • G. Watanabe Tokyo University of Agriculture and Technology, Japan.
  • K. Taya University of Gifu, Japan.

Abstract

In order to elucidate the relationship between androgens and the function of the muskrat (Ondatra zibethicus) scented glands during the breeding season, we investigated immunolocalization of steroidogenic enzymes P450scc, 3βHSD and P450c17 in the muskrat testes and scented glands. Nine adult muskrats were obtained in March (n=3), May (n=3) and July (n=3) 2010. Steroidogenic enzymes were immunolocalized using polyclonal antisera raised against bovine adrenal P450scc, human placental 3βHSD and porcine testicular P450c17. Histologically, all types of spermatogenic cells including mature–phase spermatozoa in seminiferous tubules were observed in all testes. Glandular cells, interstitial cells, epithelial cells and excretory tubules were identified in scented glands during the breeding season. P450scc, 3βHSD and P450c17 were only identified in Leydig cells during the breeding season; P450scc and P450c17 were observed in glandular cells of scented glands, however, 3βHSD was not found in scented glands during the breeding season. These novel findings provide the first evidence showing that scented glands of the muskrats are capable of locally synthesizing androgens and androgens acting via an endocrine, autocrine or paracrine manner may play an important role in scented gland function during the breeding season.

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Author Biographies

Q. Li, Beijing Forestry University
College of Biological Science and Technology
J. Weng, Beijing Forestry University
College of Biological Science and Technology
H. Zhang, Beijing Forestry University
College of Biological Science and Technology
L. Lu, Beijing Forestry University
College of Biological Science and Technology
X. Ma, Beijing Forestry University
College of Biological Science and Technology
Q. Wang, Beijing Forestry University
College of Biological Science and Technology
H. Cao, Beijing Forestry University
College of Biological Science and Technology
S. Liu, Beijing Forestry University
College of Biological Science and Technology
M. Xu, Beijing Forestry University
College of Biological Science and Technology
Q. Weng, Beijing Forestry University
College of Biological Science and Technology
G. Watanabe, Tokyo University of Agriculture and Technology
Laboratory of Veterinary Physiology
K. Taya, University of Gifu
United Graduate School of Veterinary Sciences
Published
2011-10-19
Section
Original Papers
Supporting Agencies
Beijing Forestry University
Keywords:
androgen, immunohistochemistry, muskrat, scented gland, testes
Statistics
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How to Cite
Li, Q., Weng, J., Zhang, H., Lu, L., Ma, X., Wang, Q., Cao, H., Liu, S., Xu, M., Weng, Q., Watanabe, G., & Taya, K. (2011). Immunohistochemical evidence: testicular and scented glandular androgen synthesis in muskrats (Ondatra zibethicus) during the breeding season. European Journal of Histochemistry, 55(4), e32. https://doi.org/10.4081/ejh.2011.e32