Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-α

  • M. Markelic University of Belgrade, .
  • K. Velickovic University of Belgrade, .
  • I. Golic University of Belgrade, .
  • V. Otasevic University of Belgrade, .
  • A. Stancic University of Belgrade, .
  • A. Jankovic University of Belgrade, .
  • M. Vucetic University of Belgrade, .
  • B. Buzadzic University of Belgrade, .
  • B. Korac University of Belgrade, .
  • A. Korac | aleksandra.korac@bio.bg.ac.rs Faculty of Biology, .

Abstract

The aim of the present study was to investigate whether hyperinsulinaemia, which frequently precedes insulin resistance syndrome (obesity, diabetes), induces apoptosis of endothelial cells (ECs) in brown adipose tissue (BAT) and causes BAT atrophy and also, to investigate the possible mechanisms underlying ECs death. In order to induce hyperinsulinaemia, adult male rats of Wistar strain were treated with high dose of insulin (4 U/kg, intraperitonealy) for one or three days. Examinations at ultrastructural level showed apoptotic changes of ECs, allowing us to point out that changes mainly but not exclusively, occur in nuclei. Besides different stages of condensation and alterations of the chromatin, nuclear fragmentation was also observed. Higher number of ECs apoptotic nuclei in the BAT of hyperinsulinaemic rats was also confirmed by propidium iodide staining. Immunohistochemical localization of tumor necrosis factor-alpha (TNF-α) revealed increased expression in ECs of BAT of hyperinsulinaemic animals, indicating its possible role in insulin-induced apoptotic changes. These results suggest that BAT atrophy in hyperinsulinaemia is a result of endothelial and adipocyte apoptosis combined, rather than any of functional components alone.

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Author Biographies

M. Markelic, University of Belgrade
Faculty of Biology, Centre for Electron Microscopy
K. Velickovic, University of Belgrade
Faculty of Biology, Centre for Electron Microscopy
I. Golic, University of Belgrade
Faculty of Biology, Centre for Electron Microscopy
V. Otasevic, University of Belgrade
Institute for Biological Research “Sinisa Stankovic"
A. Stancic, University of Belgrade
Institute for Biological Research “Sinisa Stankovic"
A. Jankovic, University of Belgrade
Institute for Biological Research “Sinisa Stankovic"
M. Vucetic, University of Belgrade
Institute for Biological Research “Sinisa Stankovic"
B. Buzadzic, University of Belgrade
Institute for Biological Research “Sinisa Stankovic"
B. Korac, University of Belgrade
Institute for Biological Research “Sinisa Stankovic"
A. Korac, Faculty of Biology
Faculty of Biology, Centre for Electron Microscopy
Published
2011-11-04
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Section
Original Papers
Keywords:
insulin, TNF-alpha, apoptosis, endothelial cell, brown adipose tissue
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How to Cite
Markelic, M., Velickovic, K., Golic, I., Otasevic, V., Stancic, A., Jankovic, A., Vucetic, M., Buzadzic, B., Korac, B., & Korac, A. (2011). Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-α. European Journal of Histochemistry, 55(4), e34. https://doi.org/10.4081/ejh.2011.e34