Decreased apoptosis in fatty livers submitted to subnormothermic machine-perfusion respect to cold storage

  • E. Boncompagni | boncompa@unipv.it Department of Animal Biology and Histochemistry and Cytometry Section IGM-CNR, Pavia University, Italy.
  • E. Gini Department of Animal Biology and Histochemistry and Cytometry Section IGM-CNR, Pavia University, Italy.
  • A. Ferrigno Department of Internal Medicine and Therapeutics; Pavia University, Italy.
  • G. Milanesi Department of Animal Biology and Histochemistry and Cytometry Section IGM-CNR, Pavia University, Italy.
  • E. Gringeri Department of Surgical and Gastroenterological Sciences P.G. Cevese, University of Padua, Italy.
  • S. Barni Department of Animal Biology and Histochemistry and Cytometry Section IGM-CNR, Italy.
  • U. Cillo Department of Surgical and Gastroenterological Sciences P.G. Cevese, University of Padua, Italy.
  • M. Vairetti Department of Internal Medicine and Therapeutics; Pavia University, Italy.
  • I. Freitas Department of Animal Biology and Histochemistry and Cytometry Section IGM-CNR, Pavia University, Italy.

Abstract

Machine perfusion at subnormothermic temperature (20°C), MP20, was developed by Vairetti et al. and showed to afford a better preservation of fatty livers respect to traditional cold storage (CS) in terms of enzyme release into the perfusate and bile, glycogen stores, energy charge and oxidative stress. Here we investigated whether it also caused decreased cell death by apoptosis. Fatty and lean Zucker rats were submitted to MP20 or CS for 6 h and reperfused normothermically for 2 h. Apoptotic cells were revealed by immunohistochemistry of activated caspase-3 and M30 (new epitope on CK18 degraded by caspase-3) and by the TUNEL assay. Portal pressure was also determined. A statistically significant reduction of hepatocyte apoptosis, but especially of sinusoidal cells was determined for fatty livers submitted to MP20 respect to CS. Portal pressure was significantly lower after MP20 respect to CS. The reduction of sinusoidal cell death by apoptosis without need for anti-apoptotic therapies appears particularly positive since apoptotic sinusoidal cells hinder microcirculation in the sinusoids and are thrombogenic. These results further confirm the potential of MP20 for preserving fatty livers that would be otherwise discarded as grafts, and thus for increasing the donor pool for liver transplantation.

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Published
2011-11-18
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Original Papers
Supporting Agencies
MIUR-PRIN 2004 and 2006 and by F.A.R.- University of Pavia.
Keywords:
fatty liver, transplantation, apoptosis, sinusoidal cells, sub-normothermic machine perfusion, cold storage
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How to Cite
Boncompagni, E., Gini, E., Ferrigno, A., Milanesi, G., Gringeri, E., Barni, S., Cillo, U., Vairetti, M., & Freitas, I. (2011). Decreased apoptosis in fatty livers submitted to subnormothermic machine-perfusion respect to cold storage. European Journal of Histochemistry, 55(4), e40. https://doi.org/10.4081/ejh.2011.e40