Immunohistochemical and ultrastructural visualization of different routes of oocyte elimination in adult rats

  • M.L. Escobar Sánchez Facultad de Ciencias, UNAM, Mexico.
  • O.M. Echeverría Martínez Facultad de Ciencias, UNAM, Mexico.
  • G.H. Vázquez-Nin | vazqueznin@ciencias.unam.mx Facultad de Ciencias, UNAM, Mexico.

Abstract

Cell death is a process for maintaining homeostasis in tissues and organs. In the ovary, apoptotic cell death has been implicated in follicular atresia; in the elimination of the follicles that are not ovulated during adult life. Recent studies indicate that apoptosis and autophagy are two programmed processes of cell death. Apoptosis is performed by proteases called caspases and leads to such morphological traits as DNA fragmentation. Autophagy, in turn, is characterized by the exacerbated formation of autophagosomes; a process in which the amount of the LC3 and Lamp 1 proteins increases. In this study, oocytes from all stages of the estrous cycle of Wistar rats were analyzed. The apoptosis process was identified by immunodetecting active Caspase-3 and locating DNA fragmentation using the TUNEL technique. Autophagy was evaluated through immunodetection of the LC3 and Lamp 1 proteins, and by ultrastructural localization of autophagic vesicle formation. All techniques were conducted using the same oocytes. Results show that all phases of the estrous cycle contain dying oocytes that test positive simultaneously for apoptosis and autophagy markers. The highest level of apoptosis was found during estrus; while the proestrous stage had the highest level of autophagy. The diestrous and metestrous phases were characterized by a high frequency of the presence of markers of apoptosis and autophagy in the same oocyte. Our results demonstrate that during oocyte elimination in adult rats the proteins involved in both processes, apoptosis and autophagy, are present in the same cell at the same time.

Downloads

Download data is not yet available.

Author Biographies

M.L. Escobar Sánchez, Facultad de Ciencias, UNAM
Departamento de Biología Celular
O.M. Echeverría Martínez, Facultad de Ciencias, UNAM
Departamento de Biología Celular
G.H. Vázquez-Nin, Facultad de Ciencias, UNAM
Departamento de Biología Celular
Published
2012-04-16
Section
Original Papers
Supporting Agencies
CONACyT and PAPIIT IN212912-2.
Keywords:
atresia, apoptosis, autophagy, oocyte, estrous cycle.
Statistics
Abstract views: 720

PDF: 248
HTML: 8999
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Escobar Sánchez, M., Echeverría MartínezO., & Vázquez-Nin, G. (2012). Immunohistochemical and ultrastructural visualization of different routes of oocyte elimination in adult rats. European Journal of Histochemistry, 56(2), e17. https://doi.org/10.4081/ejh.2012.17