Immunohistochemical localization and morphometry of somatotrophs and lactotrophs in protein, probiotic and symbiotic supplemented molted layers

  • H. Anwar | hasib_1@hotmail.com Department of Physiology and Pharmacology, University of Agriculture, Faisalabad, Pakistan., Pakistan.
  • Z.U. Rahman Department of Physiology and Pharmacology, University of Agriculture, Faisalabad, Pakistan., Pakistan.
  • F. Muhammad University of Agriculture,, Pakistan.
  • I. Javed University of Agriculture, Pakistan.

Abstract

Two-hundred Single Comb White Leg-Horn spent hens at the age of 70 weeks were purchased from a commercial layer farm. The birds were shifted to the Poultry Research Station, Department of Physiology and Pharmacology, University of Agriculture, Faisalabad, Pakistan. High dietary zinc (3g/kg feed/day) was used to induce molting in all the birds after one week of acclimatization. Thereafter, birds were divided in groups of 50 birds each, with the following treatments: G1 [control; crude protein (CP)16%, no other supplement], G2 (CP18%, no other supplement), G3 (CP16%, Symbiotic, 85 mg/L drinking water) and G4 (CP16%, Probiotic, 85mg/L in drinking water). Fifteen birds were slaughtered at 5% of peak of production each to collect their pituitary glands. The better egg production was seen in all the supplemented groups as compared to the control. Especially an earlier post molt production recovery and delayed decline was seen in the G2 as compared to all other groups. The immunohistochemistry of the pituitary gland reveals the decrease (P≤0.01) in the cell and nucleus size as well as area of somatotrophs in G2 and G4 as compared to G1. The cell and nucleus size as well as area of lactotrophs decreased (P≤0.01) in G2, G3 and G4 as compared to G1. The better and earlier post molt production in G2 highlights the potential role of protein supplementation in connection with the decreased lactotroph size and area in molted birds.

Downloads

Download data is not yet available.

Author Biographies

H. Anwar, Department of Physiology and Pharmacology, University of Agriculture, Faisalabad, Pakistan.

PhD Scholar,

Department of Physiology and Pharmacology

Z.U. Rahman, Department of Physiology and Pharmacology, University of Agriculture, Faisalabad, Pakistan.

Professor,

Department of Physiology and Pharmacology

F. Muhammad, University of Agriculture,

Assistant Professor

Department of Physiology and Pharmacology

I. Javed, University of Agriculture

Chairman/Professor

Department of Physiology and Pharmacology

Published
2012-06-29
Section
Original Papers
Keywords:
immunohistochemistry, somatotroph, lactotroph, molting, protein, probiotic, layers
Statistics
Abstract views: 354

PDF: 179
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Anwar, H., Rahman, Z., Muhammad, F., & Javed, I. (2012). Immunohistochemical localization and morphometry of somatotrophs and lactotrophs in protein, probiotic and symbiotic supplemented molted layers. European Journal of Histochemistry, 56(3), e28. https://doi.org/10.4081/ejh.2012.e28