Intracellular distribution of Tankyrases as detected by multicolor immunofluorescence techniques

  • M.G. Bottone Università di Pavia, Italy.
  • G. Santin Istituto di Genetica Molecolare CNR, Pavia, Italy.
  • C. Soldani Università di Pavia, Italy.
  • P. Veneroni Università di Pavia, Italy.
  • A.I. Scovassi | ivana.scovassi@gmail.com Istituto di Genetica Molecolare CNR, Pavia, Italy.
  • C. Alpini IRCCS San Matteo, Italy.

Abstract

Poly(ADP-ribose) polymerases are a family of enzymes that catalyze the conversion of NAD+ into ADP-ribose. Among them, Tankyrases have been found to bind to centrosome, mitotic spindle and microsome proteins, in the cytoplasm, and to telomeres in the nucleus, where they play a relevant role in telomere metabolism. However, their precise intracellular localization during interphase has not been so far fully elucidated. We investigated this aspect in situ by double immunofluorescence experiments using antibodies recognizing Tankyrases 1-2 or other proteins residing in specific organelles (Golgi apparatus, mitochondria, lysosomes, endoplasmic reticulum). We used HeLa cells as a model system in vitro, before and after treatment with either actinomycin D or etoposide, to also investigate the possible relocation of Tankyrases during apoptosis. We observed that Tankyrases are distributed both in the nucleus and in the cytoplasm; in this latter compartment, they were found to colocate with the Golgi apparatus but never with the mitochondria; a pool of Tankyrases also colocates with the endoplasmic reticulum and lysosomes. Interestingly, in cells with clear signs of apoptosis, Tankyrases were detectable in the cytoplasmic blebs: this suggests that they are not massively cleaved during apoptosis and persist in the largely heterogeneous apoptotic remnants which are known to contain components of cytoplasmic and nuclear origin.

Downloads

Download data is not yet available.

Author Biographies

M.G. Bottone, Università di Pavia
Dipartimento di Biologia Animale
C. Soldani, Università di Pavia
Dipartimento di Biologia Animale
P. Veneroni, Università di Pavia
Dipartimento di Biologia Animale
C. Alpini, IRCCS San Matteo
Laboratorio di Analisi Biochimico-Cliniche
Published
2012-01-20
Section
Letters to the Editor
Supporting Agencies
This work was supported by the University of Pavia (Fondo di Ateneo per la Ricerca 2010)
Keywords:
apoptosis, confocal microscopy, immunocytochemistry, organelles, PARP, Tankyrases
Statistics
Abstract views: 641

PDF: 202
HTML: 5070
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Bottone, M., Santin, G., Soldani, C., Veneroni, P., Scovassi, A., & Alpini, C. (2012). Intracellular distribution of Tankyrases as detected by multicolor immunofluorescence techniques. European Journal of Histochemistry, 56(1), e4. https://doi.org/10.4081/ejh.2012.e4