Immunological detection of m- and µ-calpains in the skeletal muscle of Marchigiana cattle

  • E. Varricchio University of Sannio., Italy.
  • M.G. Russolillo University of Sannio, Italy.
  • L. Maruccio University of Naples “Federico II”, Italy.
  • S. Velotto University of Naples “Federico II”, Italy.
  • G. Campanile University of Naples “Federico II”, Italy.
  • M. Paolucci University of Sannio, Italy.
  • F. Russo | finiziarusso@libero.it University of Sannio., Italy.

Abstract

Calpains are Ca2+-dependent proteases able to cleave a large number of proteins involved in many biological functions. Particularly, in skeletal muscle they are involved in meat tenderizing during post mortem storage. In this report we analyzed the presence and expression of µ- and m-calpains in two skeletal muscles of the Marchigiana cattle soon after slaughter, using immunocytochemical and immunohistochemical techniques, Western blotting analysis and Casein Zymography. Therefore, the presence and the activity of these proteases was investigated until 15th day post-mortem during normal process of meat tenderizing. The results showed m- and µ-calpain immunosignals in the cytoplasm both along the Z disk/I band regions and in the form of intracellular stores. Moreover, the expression level of µ-calpain but not m-calpain decreased after 10 days of storage. Such a decrease in µ-calpain was accompanied by a gradual reduction of activity. On the contrary, m-calpain activity persisted up to 15 days of post-mortem storage. Such data indicate that expression and activity of both µ-calpain and m-calpain analyzed in the Marchigiana cattle persist longer than reported in literature for other bovines and may be related to both the type of muscle and breed examined.

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Author Biographies

E. Varricchio, University of Sannio.
Department of Biological, Geological and Environmental Sciences. Via Port’Arsa, 11 , 82100 Benevento
M.G. Russolillo, University of Sannio
Centro Studi Provincia di Benevento - Contrada Piano Cappelle, 82100 Benevento - Italy
L. Maruccio, University of Naples “Federico II”

Department of Biological Structures, Functions and Technologies, , Via Veterinaria 1, 80137 Napoli

S. Velotto, University of Naples “Federico II”
Department of Soil, Plant, Environment and Animal Production,  Via Università 133 Portici (NA)
G. Campanile, University of Naples “Federico II”

Department of Animal Science and Food Inspection, Via Veterinaria 1, 80137 Napoli - Italy

M. Paolucci, University of Sannio

Department of Biological, Geological and Environmental Sciences. Via Port’Arsa, 11 , 82100 Benevento – Italy

F. Russo, University of Sannio.
Department of Biological, Geological and Environmental Sciences. Via Port’Arsa, 11 , 82100 Benevento
Published
2013-01-14
Section
Original Papers
Keywords:
m-calpain, µ-calpain, skeletal muscle, Marchigiana cattle, immunohistochemistry, Electron Microscopy.
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How to Cite
Varricchio, E., Russolillo, M., Maruccio, L., Velotto, S., Campanile, G., Paolucci, M., & Russo, F. (2013). Immunological detection of m- and µ-calpains in the skeletal muscle of Marchigiana cattle. European Journal of Histochemistry, 57(1), e2. https://doi.org/10.4081/ejh.2013.e2