In vitro reparative dentin: a biochemical and morphological study

  • G. Teti University of Bologna, Italy.
  • V. Salvatore University of Bologna, Italy.
  • A. Ruggeri University of Bologna, Italy.
  • L. Manzoli University of Bologna, Italy.
  • M. Gesi University of Pisa, Italy.
  • G. Orsini Polytechnic University of Marche, Italy.
  • M. Falconi | mirella.falconi@unibo.it University of Bologna, Italy.

Abstract

In this study, starting from human dental pulp cells cultured in vitro, we simulated reparative dentinogenesis using a medium supplemented with different odontogenic inductors. The differentiation of dental pulp cells in odontoblast-like cells was evaluated by means of staining, and ultramorphological, biochemical and biomolecular methods. Alizarin red staining showed mineral deposition while transmission electron microscopy revealed a  synthesis of extracellular matrix fibers during the differentiation process. Biochemical assays demonstrated that the differentiated phenotype expressed odontoblast markers, such as Dentin Matrix Protein 1 (DMP1) and Dentin Sialoprotein (DSP), as well as type I collagen. Quantitative data regarding the mRNA expression of DMP1, DSP and type I  collagen were obtained by Real Time PCR. Immunofluorescence data demonstrated the various localizations of DSP and DMP1 during odontoblast differentiation. Based on our results, we obtained odontoblast-like cells which simulated the reparative dentin processes in order to better investigate the mechanism of odontoblast differentiation, and dentin extracellular matrix deposition and mineralization.

 

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Published
2013-08-06
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Original Papers
Supporting Agencies
supported by the Italian Ministry of Research and Technology (MURST) with an FIRB grant (RBAP10 MLK7_005) and a PRIN 2009 grant
Keywords:
dental tissue, in vitro differentiation, DMP1, DSP, TYPE I COLLAGEN .
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How to Cite
Teti, G., Salvatore, V., Ruggeri, A., Manzoli, L., Gesi, M., Orsini, G., & Falconi, M. (2013). In vitro reparative dentin: a biochemical and morphological study. European Journal of Histochemistry, 57(3), e23. https://doi.org/10.4081/ejh.2013.e23

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