Active role of the predecidual-like zone in endometrial shedding in a mouse menstrual-like model

  • X. Xu | xiangboxuhappy@126.com National Research Institute for Family Planning, China.
  • S. Guan National Research Institute for Family Planning Graduate School of Peking Union Medical College, China.
  • B. He National Research Institute for Family Planning, China.
  • J. Wang National Research Institute for Family Planning, China.

Abstract

Cyclic shedding of the endometrium is unique to menstruating species. The status of the decidua in mouse menstrual-like models seems to differ from that of the predecidua in humans before endometrial breakdown. The aim of this study was to determine how this difference in decidual status is related to endometrial breakdown. A mouse menstrual-like model was generated by pharmacological progesterone withdrawal. Histomorphological analysis and reticular fiber staining were used to evaluate endometrial status. In situ zymography was used to determine the localization of active collagenase and gelatinase. The functional endometrial layer containing the mature decidual-like zone (MDZ) and predecidual-like zone (PZ) underwent breakdown. The reticular fibers underwent disruption and fragmentation and became loose or disappeared at 12 h in the PZ, where active collagenase and gelatinase were limited. The reticular fibers were visibly reduced at 24 h in the MDZ, where active collagenase was detected. A few reticular fibers remained; however, the functional layer had sloughed into the lumen of the uterus. The results showed that reticular fibers of the PZ are actively degraded during endometrial shedding.

Downloads

Download data is not yet available.
Published
2013-09-27
Section
Original Papers
Supporting Agencies
Work supported by the National Nature Science Foundation of China (no. 30901608) and the National Science and Technology Support Program (no. 2012BAI32B05)
Keywords:
mouse menstrual-like model, predecidual-like zone, reticular fiber, gelatinase, collagenase
Statistics
Abstract views: 1625

PDF: 216
HTML: 532
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Xu, X., Guan, S., He, B., & Wang, J. (2013). Active role of the predecidual-like zone in endometrial shedding in a mouse menstrual-like model. European Journal of Histochemistry, 57(3), e25. https://doi.org/10.4081/ejh.2013.e25