Immunohistochemical demonstration of keratins in the epidermal layers of the Malayan pangolin (Manis javanica), with remarks on the evolution of the integumental scale armour

  • W. Meyer | wilfried.meyer@tiho-hannover.de University of Veterinary Medicine Hannover Foundation, Hannover, Germany.
  • M. Liumsiricharoen Kasetsart University, Bangkok, Thailand.
  • A. Suprasert Kasetsart University, Bangkok, Thailand.
  • L.G. Fleischer Technical University of Berlin, Germany.
  • M. Hewicker-Trautwein University of Veterinary Medicine Hannover Foundation, Hannover, Germany.

Abstract

Using immunohistochemistry, the study demonstrates the distribution of keratins (pan-keratin with CK1-8, 10, 14-16, 19; keratins CK1, 5, 6, 9, 10; hair keratins AE13, AE14) in the epidermis of the Malayan pangolin (Manis javanica). A varying reaction spectrum was observed for pan-keratin, with body region-dependent negative to very strong reaction intensities. The dorsolateral epidermis exhibited positive reactions only in its vital layers, whereas the abdominal epidermis showed strong positive reactions in the soft two outer strata. The single acidic and basic-to-neutral (cyto)keratins produced clear variations compared to the pan-keratin tinging. E.g., CK1 appeared in all epidermal layers of both body regions, except for the ventral stratum corneum, whereas CK5, 6, 9, 10 were restricted to the soft ventral epidermis. Here, distinctly positive reactions were confined to the stratum granulosum, except for CK6 that appeared in the soft stratum corneum. A different staining pattern was obvious for the hair keratins, i.e., positive reactions of AE13 concentrated only in the granular layer of the dorsal epidermis. In the abdominal epidermis, remarkable tinging for AE14 was visible in the stratum basale, decreasing toward the corneal layer, but was also found in the outer root sheath cells of the hair follicles in the ventral body part. Our findings are discussed related to the evolution of the horny dorsal scales of the pangolin, which may have started from the tail root, projecting forward to the head

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Author Biographies

W. Meyer, University of Veterinary Medicine Hannover Foundation, Hannover
Histology and Embryology, Prof.Dr. (former head of this department, now retired)
M. Liumsiricharoen, Kasetsart University, Bangkok
Prof.Dr., Head of the Department of Anatomy,Faculty of Veterinary Medicine, Kasetsart University,

 

 

 

A. Suprasert, Kasetsart University, Bangkok
Prof.Dr.,  Department of Anatomy, Faculty of Veterinary Medicine, Kasetsart University
L.G. Fleischer, Technical University of Berlin
Prof.Dr., former Head of the Institute of Food Technology and Center of Biotechnology (now retired)
M. Hewicker-Trautwein, University of Veterinary Medicine Hannover Foundation, Hannover
Prof.Dr., Institute for Pathology, University of Veterinary Medicine Hannover Foundation,
Published
2013-09-16
Section
Original Papers
Keywords:
keratinisation, epidermis, scale evolution, Malayan pangolin, immunohistochemistry
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How to Cite
Meyer, W., Liumsiricharoen, M., Suprasert, A., Fleischer, L., & Hewicker-Trautwein, M. (2013). Immunohistochemical demonstration of keratins in the epidermal layers of the Malayan pangolin (Manis javanica), with remarks on the evolution of the integumental scale armour. European Journal of Histochemistry, 57(3), e27. https://doi.org/10.4081/ejh.2013.e27