Continuous cyclic mechanical tension increases ank expression in endplate chondrocytes through the TGF-β1 and p38 pathway

  • H. Xu | xuhg@maemail.com.cn Wannan Medical College, China.
  • X. Zhang Wannan Medical College, China.
  • H. Wang Wannan Medical College, China.
  • Y. Zhang Wannan Medical College, China.
  • Y. Shi Washington University Medical School, United States.
  • X. Zhang Chinese Academy of Sciences & Shanghai Jiao Tong University School of Medicine, China.

Abstract

The normal ANK protein has a strong influence on anti-calcification. It is known that TGF-β1 is also able to induce extracellular inorganic pyrophosphate (ePPi) elaboration via the TGF-β1-induced ank gene expression and the mitogen-activated protein kinase (MAPK) signaling acts as a downstream effector of TGF-β1. We hypothesized that the expression of the ank gene is regulated by mechanics through TGF-β1-p38 pathway. In this study, we investigated the mechanism of short-time mechanical tension-induced ank gene expression. We found that the continuous cyclic mechanical tension (CCMT) increased the ank gene expression in the endplate chondrocytes, and there was an increase in the TGF-β1 expression after CCMT stimulation. The ank gene expression significantly increased when treated by TGF-β1 in a dose-dependent manner and decreased when treated by SB431542 (ALK inhibitor) in a dose-dependent manner. Our study results indicate that CCMT-induced ank gene expressions may be regulated by TGF-β1 and p38 MAPK pathway.

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Author Biographies

H. Xu, Wannan Medical College
Department of Spine Surgery, Yijishan Hospital
X. Zhang, Wannan Medical College
Department of Spine Surgery, Yijishan Hospital
H. Wang, Wannan Medical College
Department of Spine Surgery, Yijishan Hospital
Y. Zhang, Wannan Medical College
Department of Spine Surgery, Yijishan Hospital
Y. Shi, Washington University Medical School
Department of Medicine
X. Zhang, Chinese Academy of Sciences & Shanghai Jiao Tong University School of Medicine
Key Laboratory of Stem Cell Biology
Published
2013-09-25
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Section
Original Papers
Supporting Agencies
Chinese national natural sciences fund project (30973025, 81272048), Chinese Anhui Province Education Department Key Fund Project (KJ2010A320).
Keywords:
Continuous cyclic mechanical tension (CCMT), Endplate chondrocytes, ank, TGF-β1, p38.
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How to Cite
Xu, H., Zhang, X., Wang, H., Zhang, Y., Shi, Y., & Zhang, X. (2013). Continuous cyclic mechanical tension increases ank expression in endplate chondrocytes through the TGF-β1 and p38 pathway. European Journal of Histochemistry, 57(3), e28. https://doi.org/10.4081/ejh.2013.e28