Dentin matrix protein 1 and dentin sialophosphoprotein in human sound and carious teeth: an immunohistochemical and colorimetric assay

  • D. Martini University of Bologna, Italy.
  • A. Trirè University of Bologna, Italy.
  • L. Breschi University of Trieste, Italy.
  • A. Mazzoni University of Trieste, Italy.
  • G. Teti University of Bologna, Italy.
  • M. Falconi University of Bologna, Italy.
  • A. Ruggeri Jr | alessandra.ruggeri@unibo.it

Abstract

Dentin matrix protein 1 (DMP1) and dentin sialophosphoprotein (DSPP) are extracellular matrix proteins produced by odontoblasts involved in the dentin mineralization. The aim this study was to compare the distribution of DMP1 and DSPP in human sound dentin vs human sclerotic dentin. Sixteen sound and sixteen carious human molars were selected, fixed in paraformaldehyde and processed for immunohistochemical detection of DMP1 and DSPP by means of light microscopy, transmission electron microscopy (TEM) and high-resolution field emission in-lens scanning electron microscopy (FEI-SEM). Specimens were submitted to a pre-embedding or a post-embedding immunolabeling technique using primary antibodies anti DMP1 and anti-DSPP and gold-conjugated secondary antibodies. Other samples were processed for the detection of DMP1 and DSPP levels. Dentin from these samples was mechanically fractured to powder, then a protein extraction and a protein level detection assay were performed. DMP1 and DSPP were more abundant in carious than in sound samples. Immunohistochemical analyses in sclerotic dentin disclosed a high expression of DMP1 and DSPP inside the tubules, suggesting an active biomineralization of dentin by odontoblasts. Furthermore, the detection of small amounts of these proteins inside the tubules far from the carious lesion, as shown in the present study, is consistent with the hypothesis of a preventive defense of all dentin after a noxious stimulus has undermined the tooth.

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Author Biographies

D. Martini, University of Bologna
Department of Biomedical and Neuromotor Sciences
A. Trirè, University of Bologna
Department of Biomedical and Neuromotor Sciences
L. Breschi, University of Trieste
Department of Medical Sciences, Unit of Dental Sciences and Biomaterials
A. Mazzoni, University of Trieste
Department of Medical Sciences, Unit of Dental Sciences and Biomaterials
G. Teti, University of Bologna
Department of Biomedical and Neuromotor Sciences
M. Falconi, University of Bologna
Department of Biomedical and Neuromotor Sciences
A. Ruggeri Jr
Department of Biomedical and Neuromotor Sciences
Published
2013-10-29
Section
Original Papers
Keywords:
sclerotic dentin, dentin matrix protein, dentin sialophosphoprotein, immunohistochemistry.
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How to Cite
Martini, D., TrirèA., Breschi, L., Mazzoni, A., Teti, G., Falconi, M., & Ruggeri Jr, A. (2013). Dentin matrix protein 1 and dentin sialophosphoprotein in human sound and carious teeth: an immunohistochemical and colorimetric assay. European Journal of Histochemistry, 57(4), e32. https://doi.org/10.4081/ejh.2013.e32