Correlation between PARP-1 immunoreactivity and cytomorphological features of parthanatos, a specific cellular death in breast cancer cells

  • P. Donizy | piotrdonizy@wp.pl Wroclaw Medical University, Poland.
  • A. Halon Wroclaw Medical University, Poland.
  • P. Surowiak Wroclaw Medical University, Poland.
  • G. Pietrzyk 4th Military Hospital Wroclaw, Poland.
  • C. Kozyra Wroclaw University of Economics, Poland.
  • R. Matkowski Lower Silesian Oncology Centre Wroclaw, Poland.

Abstract

In parthanatos, a PARP-1 (poly (ADP-ribose) polymerase 1)-mediated cell death, dissipation of mitochondrial membrane potential, large-scale DNA fragmentation and chromatin condensation were observed. In contrast to apoptosis, it does not cause apoptotic bodies formation. Although PARP-1-mediated cell death presents loss of membrane integrity similar to necrosis, it does not induce cell swelling. The purpose of the study was to correlate the immunohistochemical parameters of PARP-1 reactivity and the selected cytomorphological features of parthanatos: the lack of apoptotic bodies and the absence of necrosis in breast cancer (BC) specimens. Immunohistochemistry for PARP-1 was performed on 83 BC specimens. Correlations between parameters of PARP-1 expression and sub-cellular localisation and the presence of apoptotic bodies and necrosis were evaluated. High expression of PARP-1 (ImmunoReactive Score ≥6) was associated with the lack of apoptotic bodies (P=0.013) and with the absence of necrosis (P=0.002). The presence of apoptotic bodies was correlated with re-distribution of PARP-1 from the nucleus to cytoplasm in BC cells (P=0.029). Additionally, a tendency was observed between necrosis and loss of nuclear PARP-1 expression (P=0.049). Our study suggests that PARP-1 may play a crucial role in induction and regulation of specific type of cellular death called parthanatos.

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Author Biographies

P. Donizy, Wroclaw Medical University
Department of Pathomorphology and Oncological Cytology
A. Halon, Wroclaw Medical University
Department of Pathomorphology and Oncological Cytology
P. Surowiak, Wroclaw Medical University
Department of Histology and Embryology
G. Pietrzyk, 4th Military Hospital Wroclaw
Laboratory of Mammotomic Biopsy
C. Kozyra, Wroclaw University of Economics
Department of Statistics
Published
2013-11-20
Section
Brief Reports
Supporting Agencies
the study was supported by the grant ST-594 of Wroclaw Medical University.
Keywords:
parthanatos, cell death, PARP-1, immunohistochemistry, breast cancer.
Statistics
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How to Cite
Donizy, P., Halon, A., Surowiak, P., Pietrzyk, G., Kozyra, C., & Matkowski, R. (2013). Correlation between PARP-1 immunoreactivity and cytomorphological features of parthanatos, a specific cellular death in breast cancer cells. European Journal of Histochemistry, 57(4), e35. https://doi.org/10.4081/ejh.2013.e35