@article{Lee_Anderson_Stevens_Beasley_Barnett_Pow_2013, title={Excitatory Amino Acid Transporter 5 is widely expressed in peripheral tissues}, volume={57}, url={https://www.ejh.it/ejh/article/view/ejh.2013.e11}, DOI={10.4081/ejh.2013.e11}, abstractNote={It is routinely stated in the literature that Excitatory Amino Acid Transporter 5 (EAAT5) is a retina-specific glutamate transporter. EAAT5 is expressed by retinal photoreceptors and bipolar cells, where it serves as a slow transporter and as an inhibitory glutamate receptor, the latter role is due to the gating of a large chloride conductance. The dogma of an exclusively retinal distribution has arisen because Northern blot analyses have previously shown only modest hybridisation in non-retinal tissues. Others have re-interpreted this as indicating that EAAT5 was only present in retinal tissues. However, this view appears to be erroneous; recent evidence demonstrating abundant expression of EAAT5 in rat testis prompted us to re-examine this dogma. A new antibody was developed to an intracellular loop region of rat EAAT5. This new tool, in concert with RT-PCR and sequencing, demonstrated that EAAT5 is widely distributed at the mRNA and protein levels in many non-nervous tissues including liver, kidney, intestine, heart, lung, and skeletal muscle. We conclude that EAAT5 is a widely distributed protein. Whether it functions in all locations as a glutamate transporter, or mainly as a glutamate-gated chloride conductance, remains to be determined.}, number={1}, journal={European Journal of Histochemistry}, author={Lee, A. and Anderson, A.R. and Stevens, M. and Beasley, S. and Barnett, N.L. and Pow, D.V.}, year={2013}, month={Mar.}, pages={e11} }