@article{Caocci_Greco_Fanni_Senes_Littera_Lai_Risso_Carcassi_Faa_La Nasa_2016, title={HLA-G expression and role in advanced-stage classical Hodgkin lymphoma}, volume={60}, url={https://www.ejh.it/ejh/article/view/2606}, DOI={10.4081/ejh.2016.2606}, abstractNote={<p>Non-classical human leucocyte antigen (HLA)-G class I molecules have an important role in tumor immune escape mechanisms. We investigated HLA-G expression in lymphonode biopsies taken from 8 controls and 20 patients with advanced-stage classical Hodgkin lymphoma (cHL), in relationship to clinical outcomes and the HLA-G 14-basepair (14-bp) deletion-insertion (del-ins) polymorphism. Lymphnode tissue sections were stained using a specific murine monoclonal HLA-G antibody. HLA-G protein expression was higher in cHL patients than controls. In the group of PET-2 positive (positron emission tomography carried out after 2 cycles of standard chemotherapy) patients with a 2-year progression-free survival rate (PFS) of 40%, we observed high HLA-G protein expression within the tumor microenvironment with low expression on Hodgkin and Reed-Sternberg (HRS) cells. Conversely, PET-2 negative patients with a PFS of 86% had higher HLA-G protein expression levels on HRS cells compared to the microenvironment. Lower expression on HRS cells was significantly associated with the HLA-G 14-bp ins/ins genotype. These preliminary data suggest that the immunohistochemical pattern of HLA-G protein expression may represent a useful tool for a tailored therapy in patients with cHL, based on the modulation of HLA-G expression in relation to achievement of negative PET-2.These preliminary data suggest that the immunohistochemical pattern of HLA-G protein expression may represent a useful tool for a tailored therapy in patients with cHL, based on the modulation of HLA-G expression in relation to achievement of negative PET-2.</p>}, number={2}, journal={European Journal of Histochemistry}, author={Caocci, G. and Greco, M. and Fanni, D. and Senes, G. and Littera, R. and Lai, S. and Risso, P. and Carcassi, C. and Faa, G. and La Nasa, G.}, year={2016}, month={Apr.} }