@article{Pimentel Neto_Rocha_dos Santos Jacob_Klein Barbosa_Ciena_2021, title={Postsynaptic cleft density changes with combined exercise protocols in an experimental model of muscular hypertrophy}, volume={65}, url={https://www.ejh.it/ejh/article/view/3274}, DOI={10.4081/ejh.2021.3274}, abstractNote={<p>The vertical ladder-based protocols contribute to the NMJ junction’s adaptations, and when combined with and without load, can be potentiated. The present study aimed to investigate postsynaptic regions of the biceps brachii muscle in adult male <em>Wistar</em> rats submitted to different vertical ladder-based protocols (Sedentary - S; Climbing - C; Climbing with Load - LC and Combined Climbing - CC). The protocols (C, LC, CC) were performed in 24 sessions, 3 x/week, for 8 weeks. The myofibrillar ATPase analysis showed an increase in cross-sectional area (CSA) of the muscle fibers Type I in all trained Groups; Type II in C and LC and reduction in CC; Type IIx higher in all trained Groups. In the postsynaptic cleft, the stained area presents smaller in Groups C, LC, and CC; the total area showed smaller than LC and higher in C and CC. The stained and total perimeter, and dispersion showed a reduction in C, LC, and CC, higher maximum diameter in Groups C and CC, and decreased in LC. Regarding the postsynaptic cleft distribution, the stained area presented a decrease in all trained Groups. The integrated density presented higher principally in CC. The NMJ count showed an increase in all trained Groups. We concluded that the vertical ladder-based protocols combined contributed to the postsynaptic region adaptations.</p>}, number={s1}, journal={European Journal of Histochemistry}, author={Pimentel Neto, Jurandyr and Rocha, Lara Caetano and dos Santos Jacob, Carolina and Klein Barbosa, Gabriela and Ciena, Adriano Polican}, year={2021}, month={Aug.} }