TY - JOUR AU - Teti, G. AU - Salvatore, V. AU - Ruggeri, A. AU - Manzoli, L. AU - Gesi, M. AU - Orsini, G. AU - Falconi, M. PY - 2013/08/06 Y2 - 2024/03/28 TI - In vitro reparative dentin: a biochemical and morphological study JF - European Journal of Histochemistry JA - Eur J Histochem VL - 57 IS - 3 SE - Original Papers DO - 10.4081/ejh.2013.e23 UR - https://www.ejh.it/ejh/article/view/ejh.2013.e23 SP - e23 AB - <p class="Stile">In this study, starting from human dental pulp cells cultured <em>in vitro</em>, we simulated reparative dentinogenesis using a medium supplemented with different odontogenic inductors. The differentiation of dental pulp cells in odontoblast-like cells was evaluated by means of staining, and ultramorphological, biochemical and biomolecular methods. Alizarin red staining showed mineral deposition while transmission electron microscopy revealed a  synthesis of extracellular matrix fibers during the differentiation process. Biochemical assays demonstrated that the differentiated phenotype expressed odontoblast markers, such as Dentin Matrix Protein 1 (DMP1) and Dentin Sialoprotein (DSP), as well as type I collagen. Quantitative data regarding the mRNA expression of DMP1, DSP and type I  collagen were obtained by Real Time PCR. Immunofluorescence data demonstrated the various localizations of DSP and DMP1 during odontoblast differentiation. Based on our results, we obtained <em>odontoblast-like </em>cells which simulated the reparative dentin processes in order to better investigate the mechanism of odontoblast differentiation, and dentin extracellular matrix deposition and mineralization.</p><p> </p> ER -