TY - JOUR AU - Cortés Gutiérrez, Elva I. AU - Garcí­a-Vielma, Catalina AU - Aguilar-Lemarroy, Adriana AU - Vallejo-Ruí­z, Veronica AU - Piña-Sánchez, Patricia AU - Zapata-Benavides, Pablo AU - Gosalvez, Jaime PY - 2017/06/15 Y2 - 2024/03/29 TI - Expression of the HPV18/E6 oncoprotein induces DNA damage JF - European Journal of Histochemistry JA - Eur J Histochem VL - 61 IS - 2 SE - Original Papers DO - 10.4081/ejh.2017.2773 UR - https://www.ejh.it/ejh/article/view/2773 SP - AB - <p>This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the <em>HPV/E6</em> oncogene compared with HeLa cells with normal expression of the <em>E6</em> oncogene using the DNA breakage detection-fluorescence<em> in situ </em>hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the <em>HPV18/E6</em> oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the <em>E6</em> oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the <em>HPV/E6</em> oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.</p> ER -