TY - JOUR AU - Pellicciari, Carlo PY - 2017/12/22 Y2 - 2024/03/29 TI - Histochemistry today: Detection and location of single molecules JF - European Journal of Histochemistry JA - Eur J Histochem VL - 61 IS - 4 SE - Views and Comments DO - 10.4081/ejh.2017.2885 UR - https://www.ejh.it/ejh/article/view/2885 SP - AB - <p>Especially in the latest years, histochemical investigations have progressively been oriented toward the visualization and quantitative assessment of single molecules, thanks to the availability of stains, reactions and procedures allowing to detect <em>in situ</em> proteins, or carboydrates or nucleic acid sequences with high specificity. This is evident from the recent literature, where in the large majority of the published articles immunohistochemistry, lectin histochemistry or fluorescence <em>in situ</em> hybridization were used as experimental methodologies. Since in biomedical research it is crucial to specifically label and localize molecules there, where they exert their structural roles and activities, histochemistry will continue to provide scientists the most appropriate tools for tracing molecular maps suitable for reaching a mechanistic explanation of cell functions in tissues.</p> ER -