TY - JOUR AU - Dall'Aglio, Cecilia AU - Scocco, Paola AU - Maranesi, Margherita AU - Petrucci, Linda AU - Acuti, Gabriele AU - De Felice, Elena AU - Mercati, Francesca PY - 2019/05/03 Y2 - 2024/03/29 TI - Immunohistochemical identification of resistin in the uterus of ewes subjected to different diets: Preliminary results JF - European Journal of Histochemistry JA - Eur J Histochem VL - 63 IS - 2 SE - Brief Reports DO - 10.4081/ejh.2019.3020 UR - https://www.ejh.it/ejh/article/view/3020 SP - AB - <p>Resistin is a polypeptide hormone of the adipokine-family, primarily, but not exclusively, produced by the adipose tissue. Recent studies suggested that resistin may affect the male and female reproductive activity. The study aim was to immunohistochemically evaluate the presence and distribution of resistin in the ovine uterus. Uterine samples were collected from two groups of ewes at the end of an experimental trial during which the animals of the first group (CTRL) were fed only by grazing while those of the second one (EXP) were supplemented with barley and corn. Using a monoclonal antibody against resistin, tested by Western Blot, the immunopositive reaction was identified in the cytoplasm of epithelial lining cells and uterine glands. The endogenous production of resistin seemed to be affected by different diet, as evidenced by staining differences between the CTRL and EXP groups. Our findings support the existence of a peripheral resistin system in the sheep uterus. It is possible that this system is involved in the functionality of the uterus, which is also affected by the animal’s nutritional status.&nbsp;</p> ER -