TY - JOUR AU - Li, Ling AU - Lin, Yongqiong AU - Yang, Fang AU - Zou, Tongdan AU - Yang, Jialiang AU - Zhang, Houbin PY - 2020/09/03 Y2 - 2024/03/29 TI - An improved method for preparation of mouse retinal cryosections JF - European Journal of Histochemistry JA - Eur J Histochem VL - 64 IS - 3 SE - Brief Reports DO - 10.4081/ejh.2020.3154 UR - https://www.ejh.it/ejh/article/view/3154 SP - AB - <p class="p1">Immunohistochemistry using mouse retinal cryosections is a routine assay used in vision research. However, retinal tissues are fragile, and it is difficult to obtain an ideal retinal cryosection. Here, we developed a modified&nbsp;method for preparing retinal cryosection. Super Glue was applied on the surface of the sclera before the cornea and the lens are removed from either the unfixed or PFA-fixed mouse eyeballs. The new methods largely prevented&nbsp;retinal detachment in mouse retinal cryosections. Immunostaining of retinal cryosections derived from PFA-fixed mouse eyes using rod and cone markers yielded high-quality immunofluorescent images.&nbsp;Immunolabeling of retinal cryosections obtained from unfixed mouse eyes using a cilium-specific marker had improved orientations of photoreceptor connecting cilia. This new method substantially improves the morphology and immunostaining results of fixed and unfixed mouse eyes.</p> ER -