European Journal of Histochemistry 2019-08-06T18:18:18+02:00 Nadia Moscato Open Journal Systems <p>The <strong>European Journal of Histochemistry&nbsp;</strong>has been an influential cytology journal for over 60 years, publishing research articles on functional cytology and histology in animals and plants. The&nbsp;<strong>European Journal of Histochemistry&nbsp;</strong>offers original research articles investigating on structural and molecular components performed by histochemical and immunohistochemical methods, at light and electron microscopy, cytometry and imaging techniques.</p> <p>Areas of particular interest include cell differentiation, senescence and death, and cell-cell interactions in normal and pathological tissues; attention is also given to articles on newly developed or originally applied histochemical and microscopical techniques.</p> <p>Since its foundation in 1954,&nbsp;the <strong>European Journal of Histochemistry&nbsp;</strong>is the official organ of the Italian Society of Histochemistry.</p> Effects of physical training on sarcomere lengths and muscle-tendon interface of the cervical region in an experimental model of menopause 2019-08-06T18:18:18+02:00 Carolina dos Santos Jacob Lara Caetano Rocha Jurandyr Pimentel Neto Ii-sei Watanabe Adriano Polican Ciena <p>The aim of this study was to describe the structural and ultrastructural aspects of the myotendinous junction (MTJ) and the proximal and distal sarcomeres of the sternomastoid of aged Wistar rats subjected to an experimental model of menopause and swimming training. A total of 20 female elderly rats were divided into the following four groups (n=5 in each group): sedentary/no-menopausal (SNM), trained/no-menopausal (TNM), sedentary/menopausal (SM), and trained/menopausal (TM). The MTJ samples were dissected and analyzed using transmission electron microscopy. We showed that the TNM Group rats exhibited changes in morphological characteristics as a consequence of physical exercise, which included an increase of 36.60% (P&lt;0.001) in the evagination length of the MTJ and a reduction in the length of the distal (77.38%) (P&lt;0.0001) and proximal (68.15%) (P&lt;0.0001) sarcomeres. The SM Group exhibited a reduction of about 275.93% (P&lt;0.001) in the muscle-tendon interface and in the lengths of distal sarcomeres (55.87%) (P&lt;0.0001) compared with SNM Group. Our results suggest that the swimming training under experimental model of menopause promoted tissue reorganization and increased muscle-tendon interaction with a drastic development in the length and thickness of the sarcoplasmatic invaginations and evaginations. In addition, the sarcomeres exhibited different lengths and a reduction in both groups subjected to swimming training.</p> 2019-08-06T00:00:00+02:00 Copyright (c) 2019 The Author(s) Comparison between Lipofectamine RNAiMAX and GenMute transfection agents in two cellular models of human hepatoma 2019-08-06T18:17:50+02:00 Clarissa Berardo Veronica Siciliano Laura G. Di Pasqua Plinio Richelmi Mariapia Vairetti Andrea Ferrigno <p>RNA interference is a powerful approach to understand gene function both for therapeutic and experimental purposes. Since the lack of knowledge in the gene silencing of various hepatic cell lines, this work was aimed to compare two transfection agents, the liposome-based Lipofectamine™ RNAiMAX and the HepG2-specific, polymer-based GenMute™, in two cellular models of human hepatoma, HepG2 and Huh7.5. In the first part, we assessed transfection efficiency of a fluorescent Cy3-labeled negative control siRNA by cell imaging analysis; we found that cells treated with GenMute present a higher uptake of the fluorescent negative control siRNA when compared to Lipofectamine RNAiMAX-transfected cells, both in HepG2 and in Huh7.5 cells. In the second part, we evaluated GAPDH silencing with the two transfection reagents by RT-PCR similar GAPDH mRNA expression after each transfection treatment. Finally, we measured cell viability by the MTT assay, observing that cells transfected with GenMute have higher viability with respect to Lipofectamine RNAiMAX-administered cells. These results suggest that GenMute reagent might be considered the most suitable transfection agent for hepatic gene silencing.</p> 2019-08-06T00:00:00+02:00 Copyright (c) 2019 The Author(s)